Does this include library prep (I'm assuming yes) or just the actual sequencing step? Does this include highly scalable methods that bring the average cost down but require many, many genomes? Does this include low-pass?

Only way this will happen is if nanopores get significantly cheaper / longer-lived, on top of way more accurate. Doesn't seem like the technology is moving fast enough.

Drawing a straight line through the "The Cost of Sequencing a Human Genome" graph shows that it would be at least 200 USD. Betting NO.

What counts as a full genome? T2T (telomere to telomere) or simply as complete as one of the earlier references like hg38? Does it have to be a phased assembly or not? Does short reads count, or rather the way the industry is moving with long-read and HiFi-based assembly? the specifications are too vague.

@AlexisWalidAhmed A reference-based approach is fine, phased assembly is not needed, nor is long-read/HiFi. This seems to me to be in line with ongoing conversations in genomics, but I would caveat this is only a field I am tangentially involved in. Does that sounds reasonable?